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title: Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum:
author: Ying Chen;  Uromi Manage Goodale;  Xu-Li Fan;  Jiang-Yun Gao
Issued Date: 2015
Abstract: Paphiopedilum spicerianum    is listed as one of the country’s Wild Plants with Extremely Small Populations (PSESP). Procedures were developed for asymbiotic seed germination and seedling development aimed at producing seedlings for reintroduction. The highest germination was achieved in RECW with a 24 h dark cycle after pretreatment with 1% NaOCl for 40 min after 30 days from germination. However, these protocorms remained white and did not develop further. Although germination was lower under the same conditions in MSCW, it resulted in healthier and greener protocorms. Of four suitable media tested to promote seedling formation, Hyponex No 1 medium with View the MathML source-naphthalene acetic acid, View the MathML source activated charcoal and 10% banana homogenate was the most effective. Advanced seedling development was seen in all six tested media during a 4 month growing period, with the highest leaf growth rate seen in the same media used for seedling formation, supplemented with View the MathML source-benzyladenine added to promote leaf growth. Fluorescein diacetate (FDA) tests on seeds showed that higher salt concentrations in the medium and longer duration of exposure to NaOCl reduce germination because of damaging effects on the testa and the embryo cells.
Source: Global Ecology and Conservation
Appears in Collections:濒危植物迁地保护与再引种研究组_期刊论文

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Recommended Citation:
Ying Chen,Uromi Manage Goodale,Xu-Li Fan,et al. Asymbiotic Seed Germination And In Vitro Seedling Development Of Paphiopedilum Spicerianum:[J]. Global Ecology And Conservation,2015,3(X):367-378.

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