|Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment|
|Mao-Sheng Chen; Bang-Zhen Pan; Gui-Juan Wang; Jun Ni; Longjian Niu; Zeng-Fu Xu
|Source Publication||BMC Plant Biology
Jatropha curcas L. is a potential biofuel plant. Application of exogenous cytokinin (6-benzyladenine, BA) on its inflorescence buds can significantly increase the number of female flowers, thereby improving seed yield. To investigate which genes and signal pathways are involved in the response to cytokinin in J. curcasinflorescence buds, we monitored transcriptional activity in inflorescences at 0, 3, 12, 24, and 48 h after BA treatment using a microarray.
We detected 5,555 differentially expressed transcripts over the course of the experiment, which could be grouped into 12 distinct temporal expression patterns. We also identified 31 and 131 transcripts in J. curcaswhose homologs in model plants function in flowering and phytohormonal signaling pathways, respectively. According to the transcriptional analysis of genes involved in flower development, we hypothesized that BA treatment delays floral organ formation by inhibiting the transcription of the A, B and E classes of floral organ-identity genes, which would allow more time to generate more floral primordia in inflorescence meristems, thereby enhancing inflorescence branching and significantly increasing flower number per inflorescence. BA treatment might also play an important role in maintaining the flowering signals by activating the transcription of GIGANTEA (GI) and inactivating the transcription of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and TERMINAL FLOWER 1b (TFL1b). In addition, exogenous cytokinin treatment could regulate the expression of genes involved in the metabolism and signaling of other phytohormones, indicating that cytokinin and other phytohormones jointly regulate flower development inJ. curcas inflorescence buds.
Our study provides a framework to better understand the molecular mechanisms underlying changes in flowering traits in response to cytokinin treatment in J. curcas inflorescence buds. The results provide valuable information related to the mechanisms of cross-talk among multiple phytohormone signaling pathways in woody plants.
Mao-Sheng Chen,Bang-Zhen Pan,Gui-Juan Wang,et al. Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment[J]. BMC Plant Biology,2014,14(318).
Mao-Sheng Chen,Bang-Zhen Pan,Gui-Juan Wang,Jun Ni,Longjian Niu,&Zeng-Fu Xu.(2014).Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment.BMC Plant Biology,14(318).
Mao-Sheng Chen,et al."Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment".BMC Plant Biology 14.318(2014).
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