Choosing a suitable solvent system for a countercurrent chromatography separation presents a challenge for many researchers. In this study, we introduce a quick method of separating a target compound from the bark ofZanthoxylum myriacanthumvar.pubescensby countercurrent chromatography. This method relies on the thin-layer chromatography based generally useful estimation of solvent systems. This paper will present how to quickly choose a suitable solvent system with a thin-layer chromatography based generally useful estimation of solvent systems working chart.O-Methyltembamide (1) was enriched by countercurrent chromatography using n-hexane/ethyl acetate/methanol/water (6:4:6:4) as the solvent system. Further purification was achieved by high-performance liquid chromatography with purities of 98.2% fromZ. myriacanthumvar.pubescens bark.
Qingfei Fan,Huanli Zhang,Huabin Hu,et al. Quick Method For Separating Target Compounds From The Bark Of Maqian (zanthoxylum Myriacanthum Var. Pubescens) By High-performance Countercurrent Chromatography[J]. Journal Of Separation Science,2016,39(20):4049-4052.