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title: Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates
author: Wang, Jiancai;  Xu, Ronghua;  Wang, Ruling;  Haque, Mohammad Enamul;  Liu, Aizhong
Issued Date: 2016
Abstract: The conversion of acetyl-CoA to malonyl-CoA by acetyl-CoA carboxylase (ACC) is the rate-limiting step in fatty acid biosynthesis. In this study, a gene coding for ACC was isolated and characterized from an oleaginous yeast, Lipomyces starkeyi. Real-time quantitative PCR (qPCR) analysis of L. starkeyi acetyl-CoA carboxylase gene (LsACC1) showed that the expression levels were upregulated with the fast accumulation of lipids. The LsACC1was co-overexpressed with the glycerol 3-phosphate dehydrogenase gene (GPD1), which regulates lipids biosynthesis by supplying another substrates glycerol 3-phosphate for storage lipid assembly, in the non-oleaginous yeast Saccharomyces cerevisiae. Further, the S. cerevisiae acetyl-CoA carboxylase (ScACC1) was transferred with GPD1 and its function was analyzed in comparison with LsACC1. The results showed that overexpressed LsACC1 and GPD1 resulted in a 63% increase in S. cerevisiae. This study gives new data in understanding of the molecular mechanisms underlying the regulation of fatty acids and lipid biosynthesis in yeasts.
Source: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Appears in Collections:分子遗传和育种研究组_期刊论文

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Recommended Citation:
Wang, Jiancai,Xu, Ronghua,Wang, Ruling,et al. Overexpression Of Acc Gene From Oleaginous Yeast Lipomyces Starkeyi Enhanced The Lipid Accumulation In Saccharomyces Cerevisiae With Increased Levels Of Glycerol 3-phosphate Substrates[J]. Bioscience Biotechnology And Biochemistry,2016,80(6):1214-1222.

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