Institutional Repository of Xishuangbanna Tropical Botanical Garden
|Alternative Title||Functional Propertiesand Physiological Effects of Bamboo Shoot Dietary Fiber|
|Place of Conferral||北京|
|Keyword||竹笋膳食纤维 功能性质 肥胖 肠道菌 胰岛素抵抗|
|Other Abstract||Dendrocalamus hamiltonii, also called sweet dragon bamboo (Xishuangbanna), sweet shoot bamboo (Chinese manual of bamboo), manwan(Dailanguage), is a large cluster bamboo in Xishuangbanna. Both the bamboo stalks and the bamboo shootscan be used.Bamboo shoot (D.hamiltonii) is rich in dietary fiber. It is worthy of exploitation due to its high nutritional value.|
In this study, dietary fiber(DF)was prepared from bamboo shoots(D. hamitonii),and the chemical composition, color, micro-structure and functional properties were evaluated. Two dietary fiberisolates (BDF and TDF) were obtained from the corresponding parts (Bbs and Tbs) of bamboo shoot(D.hamiltonii).Technological property of water-holding capacity (WHC)and oil-holding capacity (OHC)of dietary fiber from the shoots were determined.
Functional properties were determined by in vitro methods including the glucose adsorption capacity (GAC) and glucose retardation index (GRI), the effect of DF on starch in vitro digestibility (SIVD), cholesterol binging capacity and cholateabsorption capacity.The resultsindicatedthat bamboo shootfibers showed excellent WHC and OHC.The starch digestibility was delayedby DF;in contrast,GAC and GRI were not affected. Bamboo shoot fibers showed a high cholesterol binding capacity with a higher value at pH 7.0 compared to pH 2.0. Moreover, adsorption of cholatewas obvious in pH7.0.
Based on the results from in vitro experiments,a 6 weekstudy was performed, which employed C57BL/6J mice fed an isocaloricand macronutrient matched high-fat diet with different fiber types including microcrystalline cellulose, bamboo shoot fiber and several other commonly consumed fibers.Results showed that the bamboo shoot fiber exhibited the most effective in controlling mice body weight gain compared with microcrystalline cellulose, wheat fiber, soybean fiber, or inulin.Bamboo shoot fiber was showntoprevent high-fat diet induced hepatic
triglycerideand cholesterol increase, fat mass increase,adipocyte grow larger, and promotelipid discharge. In addition, bamboo shoot fiberwas alsoshownto improveHOMA-IR. But no significant effects were observed on gene expression related to fat synthesis and inflammation between bamboo shoot fiber and cellulose.
Based on the prevention effect of bambooshootfiber onhigh-fat diet induced obesity, the effect of the fiberon mice gut microbiotaby 16S rRNA gene sequencingwas studied. Compared with cellulose,loss of diversityin the gut microbiotainducedby the high-fatdiet was largely prevented by bamboo shoot fiber.In addition,bamboo shoot improved the composition of intestinal flora. Taken together, the results showed that the obesity-preventing-effect of bamboo shoot fiber may be through modulatingthe gut microbiota.
Toextend the above findings,a long-term 13weekstudywasperformed and another bamboo shoot fiber from Dendrocalamus latifloruswas used for comparision. The two bamboo shoot fibers showed similar effect in suppressing high-fatdiet induced weight gain. Moreover,insulin sensitivity was examinedbyglucose tolerance test (GTT) and insulin tolerance test (ITT).Results showed that two bamboo shoot fibersdecreased body weight gain, adipose tissue mass, and hepatic lipidaccumulation, compared withcellulose. Results from GTT and ITT indicated bamboo shoot fibersimproved the high-fat diet induced decrease in insulin sensitivity. In mice liver, skeletal muscle, and adipose tissue, phosphorylationof Aktprotein, AMPK protein, andp38protein were increased. These result suggested that bamboo shoot fibers maypromote insulin function though activation of Akt pathway,increasing protein activities of catabolism.
Key Words:bamboo shoot dietary fiber, functional property, obesity,gut microbiota, insulin resistance
|李秀芬. 竹笋膳食纤维功能性质与生理作用研究[D]. 北京. 中国科学院研究生院,2016.|
|Files in This Item:|
|李秀芬-博士学位论文.pdf（5204KB）||开放获取||CC BY-NC-SA||Application Full Text|
|Recommend this item|
|Export to Endnote|
|Similar articles in Google Scholar|
|Similar articles in Baidu academic|
|Similar articles in Bing Scholar|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.