XTBG OpenIR  > 其他
Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy
Liu, Yang; Friesen, J. Brent; Grzelak, Edyta M.; Fan, Qingfei; Tang, Ting; Duric, Kemal; Jaki, Birgit U.; McAlpine, James B.; Franzblaue, Scott G.; Chen, Shao-Nong; Pauli, Guido F.
2017
Source PublicationJOURNAL OF CHROMATOGRAPHY A
Volume1504Issue:xPages:46-100
AbstractTLC-based strategies were proposed in 1979 (Hostettmann et al.) and 2005 (Friesen & Pauli; GUESS method) to minimize the number of partitioning experiments required for countercurrent separation (CCS) solvent system selection. As semi-empirical approaches, both proposed that the K values defining the sweet spot of optimal CCS corresponded to a matching Rf value range from the silica gel TLC plate developed in the organic phase of a biphasic or a corresponding monophasic solvent system. Despite their simplicity, there has been an absence of theoretical support and a deficiency of reported experimental evidence. The present study explores the theory required to develop correlations between Rf and K. All theoretical models surmise that the optimal Rf value range should be centered at 0.5. In order to validate the feasibility of the concept of matching Rf and K values, 43 natural products and six solvent system families were investigated. Out of 62 correlations, 45 resulted in matched Rf and K values. Based on this study, practical guidelines for the TLC-based prediction strategy are provided. These approaches will equip CCS users with an updated understanding of how to apply the TLC-based solvent system selection strategy to accelerate a targeted selection of CCS conditions. (C) 2017 Elsevier B.V. All rights reserved.
Subject AreaBiochemistry & Molecular Biology ; Chemistry
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10509
Collection其他
Recommended Citation
GB/T 7714
Liu, Yang,Friesen, J. Brent,Grzelak, Edyta M.,et al. Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1504(x):46-100.
APA Liu, Yang.,Friesen, J. Brent.,Grzelak, Edyta M..,Fan, Qingfei.,Tang, Ting.,...&Pauli, Guido F..(2017).Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy.JOURNAL OF CHROMATOGRAPHY A,1504(x),46-100.
MLA Liu, Yang,et al."Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy".JOURNAL OF CHROMATOGRAPHY A 1504.x(2017):46-100.
Files in This Item:
File Name/Size DocType Version Access License
Sweet spot matching_(1009KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Yang]'s Articles
[Friesen, J. Brent]'s Articles
[Grzelak, Edyta M.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Yang]'s Articles
[Friesen, J. Brent]'s Articles
[Grzelak, Edyta M.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Yang]'s Articles
[Friesen, J. Brent]'s Articles
[Grzelak, Edyta M.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Sweet spot matching_ A thin-layer chromatography-based countercurrent solvent system selection strategy.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.