XTBG OpenIR  > 植物分子生物学研究组
POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis
Zhang, Huimin; Li, Yang; Yao, Xiani; Liang, Gang; Yu, Diqiu
2017
Source PublicationPLANT PHYSIOLOGY
Volume175Issue:1Pages:543-554
AbstractOryza sativa HEMERYTHRIN MOTIF-CONTAINING REALLY INTERESTING NEW GENE AND ZINC-FINGER PROTEIN1 (OsHRZ1) is a putative iron-binding sensor. However, it is unclear how OsHRZ1 transmits signals. In this study, we reveal that POSITIVE REGULATOR OF IRON HOMEOSTASIS1 (OsPRI1) interacts with OsHRZ1. A loss-of-function mutation to OsPRI1 increased the sensitivity of plants to Fe-deficient conditions and down-regulated the expression of Fe-deficiency-responsive genes. Yeast one-hybrid and electrophoretic mobility shift assay results suggested that OsPRI1 binds to the OsIRO2 and OsIRO3 promoters. In vitro ubiquitination experiments indicated that OsPRI1 is ubiquitinated by OsHRZ1. Cell-free degradation assays revealed that the stability of OsPRI1 decreased in wild-type roots but increased in the hrz1-2 mutant, suggesting OsHRZ1 is responsible for the instability of OsPRI1. The hrz1-2 seedlings were insensitive to Fe-deficient conditions. When the pri1-1 mutation was introduced into hrz1-2 mutants, the pri1hrz1 double mutant was more sensitive to Fe deficiency than the hrz1-2 mutant. Additionally, the expression levels of Fe-deficiency-responsive genes were lower in the hrz1pri1 double mutant than in the hrz1-2 mutant. Collectively, these results imply that OsPRI1, which is ubiquitinated by OsHRZ1, mediates rice responses to Fe deficiency by positively regulating OsIRO2 and OsIRO3 expression as part of the OsHRZ1-OsPRI1- OsIRO2/3 signal transduction cascade.
Department其他
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10593
Collection植物分子生物学研究组
Recommended Citation
GB/T 7714
Zhang, Huimin,Li, Yang,Yao, Xiani,et al. POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis[J]. PLANT PHYSIOLOGY,2017,175(1):543-554.
APA Zhang, Huimin,Li, Yang,Yao, Xiani,Liang, Gang,&Yu, Diqiu.(2017).POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis.PLANT PHYSIOLOGY,175(1),543-554.
MLA Zhang, Huimin,et al."POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis".PLANT PHYSIOLOGY 175.1(2017):543-554.
Files in This Item: Download All
File Name/Size DocType Version Access License
POSITIVE REGULATOR O(1727KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Huimin]'s Articles
[Li, Yang]'s Articles
[Yao, Xiani]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Huimin]'s Articles
[Li, Yang]'s Articles
[Yao, Xiani]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Huimin]'s Articles
[Li, Yang]'s Articles
[Yao, Xiani]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.