XTBG OpenIR  > 植物分子生物学研究组
Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana
Wang, Ce; Yao, Xiani; Yu, Diqiu; Liang, Gang
2017
Source PublicationPLANTA
Volume246Issue:3Pages:421-431
AbstractMain conclusion Expression of bHLH104 - GFP driven by the MYB72 promoter improves plants' tolerance to Fe deficiency and increases seed Fe concentrations. Iron (Fe) deficiency causes reduced crop yield and quality. In humans, Fe deficiency is directly associated with Fe-deficiency anemia. Therefore, breeding Fe-deficiency tolerant and Fe-enriched plants are an ideal approach to deal with these problems. Here, different strategies were explored to generate Fe-deficiency tolerant and Fe-enriched plants. Unexpectedly, the overexpression of Fe-deficiency responsive genes (IRT1, MYB72, and bHLH100) resulted in enhanced sensitivity to Fe deficiency, including leaf chlorosis and short roots under Fe-deficiency conditions. Next, three different types of Fe-deficiency responsive promoters (Pro(IRT1) , Pro(MYB72,) and Pro(bHLH100) ) were used to drive the expression of bHLH104-GFP fusion gene in Arabidopsis. Pro(IRT1) :bHLH104-GFP plants showed the enhanced sensitivity to Fe deficiency on Fe-deficient media and the reduced fertility in alkaline soil. In contrast, Pro(bHLH100) :bHLH104-GFP plants displayed a slight tolerance to Fe deficiency and Pro (MYB72) :bHLH104-GFP plants had a significant advantage in growth in alkaline soil, including increased root length, chlorophyll, and biomass. Further analysis revealed that the expression of Fe-deficiency responsive genes was dramatically upregulated in both Pro(MYB72) :bHLH104-GFP and Pro(bHLH100) :bHLH104-GFP plants under Fe-deficiency conditions. When grown in alkaline soil, Pro(MYB72) :bHLH104-GFP plants greatly improved the seed yield and Fe concentration. These results are fundamental for plant manipulation approaches to modify tolerance to Fe deficiency and Fe accumulation through alterations of bHLH104 gene expression.
Department其他
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10595
Collection植物分子生物学研究组
Recommended Citation
GB/T 7714
Wang, Ce,Yao, Xiani,Yu, Diqiu,et al. Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana[J]. PLANTA,2017,246(3):421-431.
APA Wang, Ce,Yao, Xiani,Yu, Diqiu,&Liang, Gang.(2017).Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana.PLANTA,246(3),421-431.
MLA Wang, Ce,et al."Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana".PLANTA 246.3(2017):421-431.
Files in This Item: Download All
File Name/Size DocType Version Access License
Fe-deficiency-induce(5670KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Ce]'s Articles
[Yao, Xiani]'s Articles
[Yu, Diqiu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Ce]'s Articles
[Yao, Xiani]'s Articles
[Yu, Diqiu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Ce]'s Articles
[Yao, Xiani]'s Articles
[Yu, Diqiu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.