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麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响
甘小青; 郭娟; 张红霞; 杜芝芝; 张萍
2016
Source Publication天然产物研究与开发
ISSN1001-6880
Volume28Issue:6Pages:833-838
Abstract本研究主要探讨麻欠精油对脂多糖(LPS)诱导的THP-1细胞氧化应激的影响。溶剂对照、地塞米松或不同浓度的麻欠精油预处理THP-1细胞后,再用LPS培养24 h,用DCFH-DA荧光探针标记细胞后流式检测细胞内活性氧ROS;用FITC Annexin V/PI双染法流式检测细胞凋亡;用Griess试剂测定NO;用比色法检测总超氧化物歧化酶(SOD) ;用Western Blot法检测NADPH p47 phox的表达。结果发现麻欠精油处理组与溶剂对照组相比,LPS诱导的ROS和NO水平显著降低、SOD水平显著增加、NADPH p47 phox蛋白表达减低,细胞凋亡降低。以上实验结果表明麻欠精油对LPS诱导的THP-1细胞的氧化应激有明显抑制作用并对细胞凋亡有保护作用。
Department其他
Language中文
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10621
Collection2012年后新成立研究组
Recommended Citation
GB/T 7714
甘小青,郭娟,张红霞,等. 麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响[J]. 天然产物研究与开发,2016,28(6):833-838.
APA 甘小青,郭娟,张红霞,杜芝芝,&张萍.(2016).麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响.天然产物研究与开发,28(6),833-838.
MLA 甘小青,et al."麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响".天然产物研究与开发 28.6(2016):833-838.
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