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title: 马比木根中喜树碱和9-甲氧基喜树碱的HPLC 分析
Other Title: HPLC analysis of camptothecin and 9-methoxycamptothecin in the roots of Nothapodytes pittosporoides
author: 白永花;  宋启示
Issued Date: 2014
Abstract: 目的: 建立测定马比木根中喜树碱和9-甲氧基喜树碱的高效液相色谱法。方法: 采用5种方法对马比木根中的喜树碱和9-甲氧基喜树碱进行提取; 运用高效液相色谱法,采用Waters Symmetry C_(18)色谱柱(4. 6 mm * 250 mm,5 mum),以甲醇-水(50∶ 50)为流动相,流速0. 8 mL·min ~(- 1),检测波长254 nm,柱温26 ℃。结果: 喜树碱和9-甲氧基喜树碱进样量分别在0. 24 ~ 4. 00 mug(r = 0. 9997)和0. 24 ~ 4. 00 mug(r = 0. 9995)范围内线性关系良好; 乙醇溶液室温浸泡提取得到的喜树碱含量(0. 944%)和9-甲氧基喜树碱含量(0. 237%)明显高于其他方法。马比木是目前发现的喜树碱类含量最高的植物。结论: 本文建立的HPLC 检测法简便、准确、灵敏度高,乙醇溶液室温浸泡提取法安全,成本低,提取率高,适用于喜树碱类提取和马比木的质量检测。
English Abstract: Objective: To establish an HPLC method for the determination of camptothecin and 9-methoxycamptothecin in the roots of Nothapodytes pittosporoides. Methods: Five extraction methods were carried out respectively in the extraction of camptothecin and 9-methoxycamptothecin from roots of N. pittosporoides. HPLC was adopted. The separation was performed on a Waters Symmetry C_(18) column(4. 6 mm * 250 mm,5 mum) at 26 ℃. The mobile phase was methanol-water (50∶ 50) with a flow rate of 0. 8 mL·min ~(-1). The detective wavelength was set at 254 nm. Results: The linear ranges for camptothecin and 9-methoxycamptothecin were 0. 24-4. 00 mug(r = 0. 9997) and0. 24-4. 00 mug(r = 0. 9995) respectively. The contents of camptothecin and 9-methoxycamptothecin of the extracts from soaking in ethanol under room temperature were 0. 944% and 0. 237% respectively,which were higher than other extraction methods. N. pittosporoides contained the highest amount of camptothecins. Conclusion: This method is simple, sensitive,and accurate,which is suitable for extraction and analysis of camptothecins,and also can be applied in the quality control of N. pittosporoides.
Department: 其他
Source: 药物分析杂志
Content Type: 期刊论文
ISSN: 0254-1793
Language: 中文
Appears in Collections:2012年后新成立研究组_期刊论文

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Recommended Citation:
白永花,宋启示. 马比木根中喜树碱和9-甲氧基喜树碱的hplc 分析[J]. 药物分析杂志,2014,34(1):100-103.
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