Institutional Repository of Xishuangbanna Tropical Botanical Garden
|Alternative Title||The effect of tropical forest fragmentation on understory arthropod diversity in Xishuangbanna Naban watershed|
|Place of Conferral||北京|
|Keyword||节肢动物 物种多样性 橡胶林 森林片段化 Metabarcoding技术|
|Other Abstract||Xishuangbanna is located in the tropical northern, where biodiversity is rich. However in recent decades, the land use of Xishuangbanna undergone tremendous changes. Large-scale primary tropical forests were gradually replaced by single-planted rubber plantation, and the remaining natural forest was isolated to many different area forest fragments, which have brought a serious threat to local biodiversity. Arthropods are one of the important components for forest ecosystems and play an important role in the structure and function of ecosystems. Therefore, it is significant to insight into the influence of forest fragmentation to arthropod community. This study was set up in Nabanhe national nature reserve of Xishuangbanna, we compared understory arthropod diversity and community composition between rubber plantations and forest fragments using traditional morphological and metabarcoding technical. We also analyze the relationship between arthropods and environmental factors, and try to understand the key factors which influenced arthropod community structure. The main research results are as follows:|
1. Based on the traditional morphological analysis, 25,205 arthropos were collected by malaise trap and ground cup method in forest fragments and rubber plantations, which belong to 27 orders. The dominant taxa groups were Collembola, Lepidoptera, Acarina and Diptera, which account for 70.22%. A total of 14,227 arthropos were indentified in forest fragments, 10,978 arthropods were collected from rubber plantations. 18 groups were collected by Malaise trap, and the dominant groups were Diptera, Lepidoptera, Collembola and Psocoptera; 27 groups were collected by groung cup, and the dominant groups were Collembola, Lepidoptera, Acarina and Diptera. In order to investigate arthropods more comprehensive, two sampling methods should be combined effectively. The ANOVA analysis showed that season and forest type had significant effects on arthropods abundance and species diversity index; Arthropods abundance was higher in dry seasons compared to rainy season. The abundance of arthropods was not effect by forest fragments area. In forest fragments, the main factors affecting the composition of arthropod community were forest fragments area, altitude and slope; the influence factors in rubber forest are altitude and slope.
2. Based on the metabarcoding technique, a total of 397 OTUSs were obtained from rubber plantations and forest fragments, which belong to 18 orders. In forest fragments, a total of 347 OTUSs were collected, and 136 OTUSs were collected from rubber plantations. (1) Compared with primary forests, rubber plantations had significant negative effect on arthropod richness; The results of nonmetric multidimensional scaling analysis (NMDS analysis) showed that the composition of arthropod community in rubber plantations and forest fragments was significantly different; Beta diversity of forest fragments is larger than that of rubber plantations; (2) Arthropod richness in large forest fragments was significantly larger than small forest fragments. NMDS analysis showed that the composition of arthropod community in large area forest fragments is significantly different from that of small forest fragments; Beta diversity between large forest fragments is smaller than small forest fragments; (3) In the landscape scale, the main factors affecting the species diversity of arthropods were nature forest cover, and the main factors affecting the composition of arthropod community were nature forest cover and slope; In local scale, the main factors influencing the diversity of arthropods in rubber were the age of rubber tree, altitude and distance to forest, and the main factors affecting the composition of arthropod community were the age of rubber tree, altitude, distance to forest and slope; In forest, the main factors influencing the diversity of arthropods were forest area, altitude, aspect, tree diversity and distance to the edge of forest, and the main factors affecting the composition of arthropod community were forest fragments area, altitude, distance to the edge of forest, slope and aspect.
3. The results of diversity and composition of arthropods in forest fragments and rubber plantations were different between morphological approach and DNA barcoding: there was no significant difference in arthropods abundance between fragment forest and rubber plantations collected by Malaise, however the richness of arthropod in the fragment forest was significantly higher than that of rubber plantations; Although the NMDS analysis of the two methods were similar, but the environmental factors which effected arthropods community were different. Metabarcoding technique can effectively evaluate the community composition and species diversity of arthropods in this study.
In conclusion, this study demonstrated that rubber planting has a significant negative impact on arthropod diversity as well as forest fragments. Large areas of forests can maintain higher biological diversity than rubber plantation. The present results provide basic data and management basis for the conservation of biodiversity in Xishuangbanna area, while demonstrating that the small forest surrounded by rubber plantations still has its value and needs to be protected.
Key Words: arthropod, biodiversity, rubber plantation, forest fragment, metabarcoding
|马占霞. 西双版纳纳版河流域热带森林片段化对林下节肢动物多样性的影响[D]. 北京. 中国科学院研究生院,2017.|
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