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De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis
Fu, Qiantang; Niu, Longjian; Chen, Mao-Sheng; Tao, Yan-Bin; Wang, Xiulan; He, Huiying; Pan, Bang-Zhen; Xu, Zeng-Fu
2018
Source PublicationJOURNAL OF PLANT PHYSIOLOGY
ISSN0176-1617
Volume221Issue:xPages:107-118
AbstractPlukenetia volubilis is a promising oilseed crop due to its seeds being rich in unsaturated fatty acids, especially alpha-linolenic acid. P. volubilis is monoecious, with separate male and female flowers on the same inflorescence. We previously reported that male flowers were converted to female flowers by exogenous cytokinin (6-benzyladenine, 6-BA) treatment in P. volubilis. To identify candidate genes associated with floral sex differentiation of P. volubilis, we performed de novo transcriptome assembly and comparative analysis on control male inflorescence buds (MIB) and female inflorescence buds (FIB) induced by 6-BA using Illumina sequencing technology. A total of 57,664 unigenes with an average length of 979 bp were assembled from 104.1 million clean reads, and 45,235 (78.45%) unigenes were successfully annotated in the public databases. Notably, Gene Ontology analyses revealed that 4193 and 3880 unigenes were enriched in the categories of reproduction and reproductive processes, respectively. Differential expression analysis identified 1385 differentially expressed unigenes between MIB and FIB, of which six unigenes related to cytokinin and auxin signaling pathways and 16 important transcription factor (TF) genes including MADS-box family members were identified. In particular, several unigenes encoding important TFs, such as homologs of CRABS CLAW, RADIALIS-like 1, RADIALIS-like 2, HECATE 2, WUSCHEL-related homeobox 9, and SUPERMAN, were expressed at higher levels in FIB than in MIB. The expression patterns of the 36 selected unigenes revealed by transcriptome analysis were successfully validated by quantitative real-time PCR. This study not only provides comprehensive gene expression profiles of P. volubilis inflorescence buds, but also lays the foundation for research on the molecular mechanism of floral sex determination in P. volubilis and other monoecious plants.
Department其他
KeywordPromising Oilseed Crop Time Quantitative Pcr Mads-box Genes Flower Development Sex Determination Arabidopsis-thaliana Silene-latifolia Ricinus-communis Expression Plants
Subject AreaPlant Sciences
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10792
Collection能源植物分子育种研究组
Corresponding AuthorXu, Zeng-Fu
Recommended Citation
GB/T 7714
Fu, Qiantang,Niu, Longjian,Chen, Mao-Sheng,et al. De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis[J]. JOURNAL OF PLANT PHYSIOLOGY,2018,221(x):107-118.
APA Fu, Qiantang.,Niu, Longjian.,Chen, Mao-Sheng.,Tao, Yan-Bin.,Wang, Xiulan.,...&Xu, Zeng-Fu.(2018).De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis.JOURNAL OF PLANT PHYSIOLOGY,221(x),107-118.
MLA Fu, Qiantang,et al."De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis".JOURNAL OF PLANT PHYSIOLOGY 221.x(2018):107-118.
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