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灯盏花ARC1基因的克隆及序列分析
陈莫; 张薇; 胡彦如; 谢世清; 范伟; 杨生超
2017
Source Publication分子植物育种
ISSN1672-416X
Volume15Issue:11Pages:4389-4395
Abstract本研究对灯盏花ARC1基因进行克隆及序列分析。根据实验室前期预测灯盏花的序列设计引物,以灯盏花花蕾为材料提取RNA作为模板,通过反转录(RT-PCR)扩增得到cDNA,作为PCR扩增的模板,将扩增的ARC1基因连接到pMD19-T上,阳性克隆经PCR检测后进行测序结果分析,得到2 199 bp的核酸序列。序列分析结果表明,该基因存在2个结构域,不存在内含子,编码733个氨基酸,其核酸序列的同源性达到64%以上,氨基酸序列同源性达61%。本研究首次从灯盏花中克隆出ARC1基因,为后期研究该基因提供理论基础。
Department其他
Subject AreaPlant Sciences (Provided By Clarivate Analytics)
Language中文
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10828
Collection其他
Recommended Citation
GB/T 7714
陈莫,张薇,胡彦如,等. 灯盏花ARC1基因的克隆及序列分析[J]. 分子植物育种,2017,15(11):4389-4395.
APA 陈莫,张薇,胡彦如,谢世清,范伟,&杨生超.(2017).灯盏花ARC1基因的克隆及序列分析.分子植物育种,15(11),4389-4395.
MLA 陈莫,et al."灯盏花ARC1基因的克隆及序列分析".分子植物育种 15.11(2017):4389-4395.
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