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Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose
Zhu, Chang-hui; Fang, Zhen; Su, Tong-chao; Li, Xing-kang; Liu, Qi-ying
2018
Source PublicationCELLULOSE
ISSN0969-0239
Volume25Issue:4Pages:2473-2485
AbstractCellulose-based biochar was prepared via ionothermal carbonization of cellulose in [Bmim]Cl with H(2)SO(4)and subsequent pyrolysis. The biochar was analyzed by a series of characterization methods, indicating that it was a kind of mesoporous carbon suitable for the adsorption of cellulase. Kinetic analysis showed that the immobilized cellulase exhibited higher affinity to carboxymethyl cellulose than free cellulase. The immobilized cellulase, at different pH and temperatures, was more stable than free cellulase. It was used to hydrolyze pretreated cellulose in [Bmim]Cl with a total reducing sugar (TRS) yield of 99.9%. The immobilized cellulase maintained activity of 74.8% after five cycles at an immobilized cellulase/cellulose weight ratio of 30:1. When the cellulose loading was increased by a factor of 5, the TRS yield decreased by only 27.5%.
Department其他
KeywordMesoporous Biochar Ionothermal Carbonization Immobilized Cellulase Kinetics Analysis Stability
Subject AreaMaterials Science ; Polymer Science
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10849
Collection生物能源研究组
Recommended Citation
GB/T 7714
Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,et al. Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose[J]. CELLULOSE,2018,25(4):2473-2485.
APA Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,Li, Xing-kang,&Liu, Qi-ying.(2018).Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose.CELLULOSE,25(4),2473-2485.
MLA Zhu, Chang-hui,et al."Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose".CELLULOSE 25.4(2018):2473-2485.
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