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Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
Fan, Qingfei; Liu, Yang; Kulakowski, Daniel; Chen, Shaonong; Friesen, J. Brent; Pauli, Guido F.; Song, Qishi
2018
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1553Issue:xPages:108-115
AbstractCountercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds. (C) 2018 Elsevier B.V. All rights reserved.
Department其他
KeywordCountercurrent Separation Mammalian Steroid Hormones Progesterone Estriol Guess Method
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10856
Collection民族植物学研究组
中国科学院西双版纳热带植物园
Recommended Citation
GB/T 7714
Fan, Qingfei,Liu, Yang,Kulakowski, Daniel,et al. Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1553(x):108-115.
APA Fan, Qingfei.,Liu, Yang.,Kulakowski, Daniel.,Chen, Shaonong.,Friesen, J. Brent.,...&Song, Qishi.(2018).Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo.JOURNAL OF CHROMATOGRAPHY A,1553(x),108-115.
MLA Fan, Qingfei,et al."Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo".JOURNAL OF CHROMATOGRAPHY A 1553.x(2018):108-115.
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