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title: Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
author: Fan, Qingfei;  Liu, Yang;  Kulakowski, Daniel;  Chen, Shaonong;  Friesen, J. Brent;  Pauli, Guido F.;  Song, Qishi
Issued Date: 2018
Abstract: Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds. (C) 2018 Elsevier B.V. All rights reserved.
Related URLs: 10.1016/j.chroma.2018.04.033
Appears in Collections:民族植物学研究组_期刊论文

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全文许可: Creative Commons 署名-非商业性使用-相同方式共享 3.0

Recommended Citation:
Fan, Qingfei,Liu, Yang,Kulakowski, Daniel,et al. Countercurrent Separation Assisted Identification Of Two Mammalian Steroid Hormones In Vitex Negundo[J]. Journal Of Chromatography A,2018,1553(X):108-115.

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