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title: Identification and expression analysis of cytokinin metabolic genes IPTs CYP735A and CKXs in the biofuel plant Jiatropha curcas
author: Cai, Li;  Zhang, Lu;  Fu, Qiantang;  Xu, Zeng-Fu
Issued Date: 2018
Abstract: The seed oil of Jatropha curcas is considered a potential bioenergy source that could replace fossil fuels. However, the seed yield of Jatropha is low and has yet to be improved. We previously reported that exogenous cytokinin treatment increased the seed yield of Jatropha. Cytokinin levels are directly regulated by isopentenyl transferase (IPT), cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A), and cytokinin oxidase/dehydrogenase (CKX). In this study, we cloned six IPT genes, one JcCYP735A gene, and seven JcCKX genes. The expression patterns of these 14 genes in various organs were determined using realtime quantitative PCR. JcIPTl was primarily expressed in roots and seeds, JcIPT2 was expressed in roots, apical meristems, and mature leaves, JcIPT3 was expressed in stems and mature leaves, JcIPT5 was expressed in roots and mature leaves, JcIPT6 was expressed in seeds at 10 days after pollination, and JcIPT9 was expressed in mature leaves. JcCYP735A was mainly expressed in roots, flower buds, and seeds. The seven JcCKX genes also showed different expression patterns in different organs of Jatropha. In addition, CK levels were detected in flower buds and seeds at different stages of development. The concentration of N-6-(Delta(2)-isopentenyl)-adenine (iP), iP-riboside, and trans-zeatin (tZ) increased with flower development, and the concentration of iP decreased with seed development, while that of tZ increased. We further analyzed the function of JcCYP735A using the CRISPR-Cas9 system, and found that the concentrations of tZ and tZ-riboside decreased significantly in the Jccyp735a mutants, which showed severely retarded growth. These findings will be helpful for further studies of the functions of cytokinin metabolic genes and understanding the roles of cytokinins in Jatropha growth and development.
Source: PEERJ
Related URLs: 10.7717/peerj.4812
Appears in Collections:能源植物分子育种研究组_期刊论文

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Recommended Citation:
Cai, Li,Zhang, Lu,Fu, Qiantang,et al. Identification And Expression Analysis Of Cytokinin Metabolic Genes Ipts Cyp735a And Ckxs In The Biofuel Plant Jiatropha Curcas[J]. Peerj,2018,6(X):-.

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