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Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids
Zhao, Dake; Shen, Yong; Shi, Yana; Shi, Xingqiao; Qiao, Qin; Zi, Shuhui; Zhao, Erqiang; Yu, Diqiu; Kennelly, Edward J.
2018
Source PublicationPHYTOCHEMISTRY
ISSN0031-9422
Volume152Issue:xPages:113-124
AbstractAconitum carmichaelii has long been used as a traditional Chinese medicine, and its processed lateral roots are known commonly as fuzi. Aconitine-type C-19-diterpenoid alkaloids accumulating in the lateral roots are some of the main toxicants of this species, yet their biosynthesis remains largely unresolved. As a first step towards understanding the biosynthesis of aconitine-type C-19-diterpenoid alkaloids, we performed de novo transcriptome assembly and analysis of rootstocks and leaf tissues of Aconitum carmichaelii by next-generation sequencing. A total of 525 unigene candidates were identified as involved in the formation of C-19-diterpenoid alkaloids, including those encoding enzymes in the early steps of diterpenoid alkaloids scaffold biosynthetic pathway, such as ent-copalyl diphosphate synthases, entkaurene synthases, kaurene oxidases, cyclases, and key aminotransferases. Furthermore, candidates responsible for decorating of diterpenoid alkaloid skeletons were discovered from transcriptome sequencing of fuzi, such as monooxygenases, methyltransferase, and BAHD acyltransferases. In addition, 645 differentially expressed genes encoding transcription factors potentially related to diterpenoid alkaloids accumulation underground were documented. Subsequent modular domain structure phylogenetics and differential expression analysis led to the identification of BAHD acyltransferases possibly involved in the formation of acetyl and benzoyl esters of diterpenoid alkaloids, associated with the acute toxicity of fuzi. The transcriptome data provide the foundation for future research into the molecular basis for aconitine-type C-19-diterpenoid alkaloids biosynthesis in A. carmichaelii. (C) 2018 Published by Elsevier Ltd.
Department其他
KeywordAconitum Carmichaelii (Ranunculaceae) Fuzi Transcriptome Biosynthesis Aconitine-type C-19-diterpenoid Alkaloids Acute Toxicity Traditional Chinese Medicine (Tcm)
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/10971
Collection植物分子生物学研究组
Recommended Citation
GB/T 7714
Zhao, Dake,Shen, Yong,Shi, Yana,et al. Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids[J]. PHYTOCHEMISTRY,2018,152(x):113-124.
APA Zhao, Dake.,Shen, Yong.,Shi, Yana.,Shi, Xingqiao.,Qiao, Qin.,...&Kennelly, Edward J..(2018).Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids.PHYTOCHEMISTRY,152(x),113-124.
MLA Zhao, Dake,et al."Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids".PHYTOCHEMISTRY 152.x(2018):113-124.
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