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Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis
Tian, Bo; Lu, Tianquan; Xu, Yang1; Wang, Ruling; Chen, Guanqun1
2019
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
ISSN1754-6834
Volume12Issue:xPages:-
Abstract

Ricinoleic acid is a high-value hydroxy fatty acid with broad industrial applications. Hiptage benghalensis seed oil contains a high amount of ricinoleic acid (similar to 80%) and represents an emerging source of this unusual fatty acid. However, the mechanism of ricinoleic acid accumulation in H. benghalensis is yet to be explored at the molecular level, which hampers the exploration of its potential in ricinoleic acid production. To explore the molecular mechanism of ricinoleic acid biosynthesis and regulation, H. benghalensis seeds were harvested at five developing stages (13, 16, 19, 22, and 25 days after pollination) for lipid analysis. The results revealed that the rapid accumulation of ricinoleic acid occurred at the early-mid-seed development stages (16-22 days after pollination). Subsequently, the gene transcription profiles of the developing seeds were characterized via a comprehensive transcriptome analysis with second-generation sequencing and single-molecule real-time sequencing. Differential expression patterns were identified in 12,555 transcripts, including 71 enzymes in lipid metabolic pathways, 246 putative transcription factors (TFs) and 124 long noncoding RNAs (lncRNAs). Twelve genes involved in diverse lipid metabolism pathways, including fatty acid biosynthesis and modification (hydroxylation), lipid traffic, triacylglycerol assembly, acyl editing and oil-body formation, displayed high expression levels and consistent expression patterns with ricinoleic acid accumulation in the developing seeds, suggesting their primary roles in ricinoleic acid production. Subsequent co-expression network analysis identified 57 TFs and 35 lncRNAs, which are putatively involved in the regulation of ricinoleic acid biosynthesis. The transcriptome data were further validated by analyzing the expression profiles of key enzyme-encoding genes, TFs and lncRNAs with quantitative real-time PCR. Finally, a network of genes associated with ricinoleic acid accumulation in H. benghalensis was established. This study was the first step toward the understating of the molecular mechanisms of ricinoleic acid biosynthesis and oil accumulation in H. benghalensis seeds and identified a pool of novel genes regulating ricinoleic acid accumulation. The results set a foundation for developing H. benghalensis into a novel ricinoleic acid feedstock at the transcriptomic level and provided valuable candidate genes for improving ricinoleic acid production in other plants.

KeywordHydroxy Fatty-acid Coa-binding Proteins Seed Oil Oleate 12-hydroxylase Physaria-fendleri Lesquerella Expression Enzyme Acyltransferase Biosynthesis
Subject AreaBiotechnology & Applied Microbiology ; Energy & Fuels
DOI10.1186/s13068-019-1358-2
Indexed BySCI
Language英语
WOS IDWOS:000458098800001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11215
Collection药用植物栽培技术组
Affiliation1.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China
2.Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada
Recommended Citation
GB/T 7714
Tian, Bo,Lu, Tianquan,Xu, Yang,et al. Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis[J]. BIOTECHNOLOGY FOR BIOFUELS,2019,12(x):-.
APA Tian, Bo,Lu, Tianquan,Xu, Yang,Wang, Ruling,&Chen, Guanqun.(2019).Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis.BIOTECHNOLOGY FOR BIOFUELS,12(x),-.
MLA Tian, Bo,et al."Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis".BIOTECHNOLOGY FOR BIOFUELS 12.x(2019):-.
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