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Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha
Zhao, Mei-Li; Ni, Jun; Chen, Mao-Sheng; Xu, Zeng-Fu
2019
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume20Issue:xPages:-
Abstract

Trehalose-6-phosphate (T6P) phosphatase (TPP), a dephosphorylating enzyme, catalyzes the dephosphorylation of T6P, generating trehalose. In Jatropha, we found six members of the TPP family. Five of them JcTPPA, JcTPPC, JcTPPD, JcTPPG, and JcTPPJ are highly expressed in female flowers or male flowers, or both, suggesting that members of the JcTPP family may participate in flower development in Jatropha. The wide expression of JcTPPJ gene in various organs implied its versatile roles and thus was chosen for unraveling its biological functions during developmental process. We constructed an overexpression vector of JcTPPJ cDNA driven by the cauliflower mosaic virus (CaMV) 35S promoter for genetic transformation. Compared with control Arabidopsis plants, 35S:JcTPPJ transgenic Arabidopsis plants presented greater sucrose contents in their inflorescences and displayed late-flowering and heterostylous phenotypes. Exogenous application of sucrose to the inflorescence buds of wild-type Arabidopsis repressed the development of the perianth and filaments, with a phenocopy of the 35S:JcTPPJ transgenic Arabidopsis. These results suggested that the significantly increased sucrose level in the inflorescence caused (or induced) by JcTTPJ overexpression, was responsible for the formation of heterostylous flower phenotype. However, 35S:JcTPPJ transgenic Jatropha displayed no obvious phenotypic changes, implying that JcTPPJ alone may not be sufficient for regulating flower development in Jatropha. Our results are helpful for understanding the function of TPPs, which may regulate flower organ development by manipulating the sucrose status in plants.

KeywordTREHALOSE 6-PHOSPHATE GENE-EXPRESSION DIFFERENT-FORMS METABOLISM BIOSYNTHESIS GROWTH TOLERANCE EVOLUTION STRESS SUGAR
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.3390/ijms20092165
Indexed BySCI
Language英语
WOS IDWOS:000469753500112
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11321
Collection能源植物分子育种研究组
Affiliation1.[Zhao, Mei-Li
2.Ni, Jun
3.Chen, Mao-Sheng
4.Chinese Acad Sci, Innovat Acad Seed Design, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Plant Resources & Sustainable Us, Mengla 666303, Yunnan, Peoples R China
5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Mei-Li,Ni, Jun,Chen, Mao-Sheng,et al. Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(x):-.
APA Zhao, Mei-Li,Ni, Jun,Chen, Mao-Sheng,&Xu, Zeng-Fu.(2019).Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(x),-.
MLA Zhao, Mei-Li,et al."Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.x(2019):-.
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