XTBG OpenIR  > 2012年后新成立研究组
Overexpression of OsAGO1b Induces Adaxially Rolled Leaves by Affecting Leaf Abaxial Sclerenchymatous Cell Development in Rice
Li, Youhan1; Yang, Yiqi; Liu, Ye1,2; Li, Dexia; Zhao, Yahuan; Li, Zhijie; Liu, Ying; Jiang, Dagang; Li, Jing; Zhou, Hai; Chen, Jianghua1; Zhuang, Chuxiong; Liu, Zhenlan3
2019
Source PublicationRICE
ISSN1939-8425
Volume12Issue:1Pages:-
Abstract

Background ARGONAUTE 1 (AGO1) proteins can recruit small RNAs to regulate gene expression, involving several growth and development processes in Arabidopsis. Rice genome contains four AGO1 genes, OsAGO1a to OsAGO1d. However, the regulatory functions to rice growth and development of each AGO1 gene are still unknown. Results We obtained overexpression and RNAi transgenic lines of each OsAGO1 gene. However, only up- and down-regulation of OsAGO1b caused multiple abnormal phenotypic changes in rice, indicating that OsAGO1b is the key player in rice growth and organ development compared with other three OsAGO1s. qRT-PCR assays showed that OsAGO1b was almost unanimously expressed in leaves at different developmental stages, and strongly expressed in spikelets at S1 to S3 stages. OsAGO1b is a typical AGO protein, and co-localized in both the nucleus and cytoplasm simultaneously. Overexpression of OsAGO1b caused adaxially rolled leaves and a series of abnormal phenotypes, such as the reduced tiller number and plant height. Knockdown lines of OsAGO1b showed almost normal leaves, but the seed setting percentage was significantly reduced accompanied by the disturbed anther patterning and reduced pollen fertility. Further anatomical observation revealed that OsAGO1b overexpression plants showed the partially defective development of sclerenchymatous cells on the abaxial side of leaves. In situ hybridization showed OsAGO1b mRNA was uniformly accumulated in P1 to P3 primordia without polarity property, suggesting OsAGO1b did not regulate the adaxial-abaxial polarity development directly. The expression levels of several genes related to leaf polarity development and vascular bundle differentiation were observably changed. Notably, the accumulation of miR166 and TAS3-siRNA was decreased, and their targeted OSHBs and OsARFs were significantly up-regulated. The mRNA distribution patterns of OSHB3 and OsARF4 in leaves remained almost unchanged between ZH11 and OsAGO1b overexpression lines, but their expression levels were enhanced at the regions of vascular bundles and sclerenchymatous cell differentiation. Conclusions In summary, we demonstrated OsAGO1b is the leading player among four OsAGO1s in rice growth and development. We propose that OsAGO1b may regulate the abaxial sclerenchymatous cell differentiation by affecting the expression of OSHBs in rice.

KeywordARGONAUTE protein Leaf rolling OsAGO1b Rice Sclerenchymatous cell Small RNA
Subject AreaAgriculture
DOI10.1186/s12284-019-0323-9
Indexed BySCI
Language英语
WOS IDWOS:000480521600001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11476
Collection2012年后新成立研究组
Affiliation1.South China Agr Univ, Coll Life Sci, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, CAS Ctr Excellence Mol Plant Sci, Key Lab Trop Plant Resources & Sustainable Use, Kunming 650223, Yunnan, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
4.South China Agr Univ, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Youhan,Yang, Yiqi,Liu, Ye,et al. Overexpression of OsAGO1b Induces Adaxially Rolled Leaves by Affecting Leaf Abaxial Sclerenchymatous Cell Development in Rice[J]. RICE,2019,12(1):-.
APA Li, Youhan.,Yang, Yiqi.,Liu, Ye.,Li, Dexia.,Zhao, Yahuan.,...&Liu, Zhenlan.(2019).Overexpression of OsAGO1b Induces Adaxially Rolled Leaves by Affecting Leaf Abaxial Sclerenchymatous Cell Development in Rice.RICE,12(1),-.
MLA Li, Youhan,et al."Overexpression of OsAGO1b Induces Adaxially Rolled Leaves by Affecting Leaf Abaxial Sclerenchymatous Cell Development in Rice".RICE 12.1(2019):-.
Files in This Item: Download All
File Name/Size DocType Version Access License
Overexpression of Os(8512KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Youhan]'s Articles
[Yang, Yiqi]'s Articles
[Liu, Ye]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Youhan]'s Articles
[Yang, Yiqi]'s Articles
[Liu, Ye]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Youhan]'s Articles
[Yang, Yiqi]'s Articles
[Liu, Ye]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Overexpression of OsAGO1b Induces Adaxially Rolled Leaves by Affecting Leaf Abaxial Sclerenchymatous Cell Development in Rice.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.