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A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis
Shan, Fangzhen; Mei, Song1; Zhang, Jiahai; Zhang, Xuecheng2; Xu, Chao; Liao, Shanhui; Tu, Xiaoming
2019
Source PublicationFEBS JOURNAL
ISSN1742-464X
Volume286Issue:16Pages:3129-3147
Abstract

The autoantigen La protein is an important component of telomerase and a predominantly nuclear phosphoprotein. As a telomerase subunit, La protein associates with the telomerase ribonucleoprotein and influences telomere length. In the reverse transcription, La protein stimulates enzymatic activity and increases repeated addition processivity of telomerase. As nuclear phosphoprotein, La protein binds the 3 ' poly(U)-rich elements of nascent RNA polymerase III transcripts to facilitate its correct folding and maturation. In this work, we identified a La protein homolog (TbLa) from Trypanosoma brucei (T. brucei). We revealed that TbLa interacts with ribosome-associated protein P34/P37, 40S ribosomal protein SA, and 60S ribosomal subunit L5 in T. brucei. In the interactions between TbLa protein and (P34/P37)/L5/SA, RNA recognition motif (RRM) domain of TbLa was indicated to make the major contribution to the processes. We determined the solution structure of TbLa RRM domain. NMR chemical shift perturbations revealed that the positively charged RNA-binding pocket of TbLa RRM domain is responsible for its interaction with ribosomal and ribosome-associated proteins P37/L5/SA. Furthermore, depletion of TbLa affected the maturation process of 5S rRNA and ribosomal assembly, suggesting TbLa protein might play a significant role in the ribosomal biogenesis pathway in T. brucei. Taken together, our results provide a novel insight and structural basis for better understanding the roles of TbLa and RRM domain in ribosomal biogenesis in T. brucei. Database Structural data are available in the PDB under the accession number .

KeywordLa protein ribosomal biogenesis RRM domain solution structure Trypanosoma brucei
Subject AreaBiochemistry & Molecular Biology
DOI10.1111/febs.14853
Indexed BySCI
Language英语
WOS IDWOS:000481765400005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11483
Collection其他
Affiliation1.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei, Anhui, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Kunming, Yunnan, Peoples R China
3.Anhui Univ, Sch Life Sci, Hefei, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Shan, Fangzhen,Mei, Song,Zhang, Jiahai,et al. A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis[J]. FEBS JOURNAL,2019,286(16):3129-3147.
APA Shan, Fangzhen.,Mei, Song.,Zhang, Jiahai.,Zhang, Xuecheng.,Xu, Chao.,...&Tu, Xiaoming.(2019).A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis.FEBS JOURNAL,286(16),3129-3147.
MLA Shan, Fangzhen,et al."A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis".FEBS JOURNAL 286.16(2019):3129-3147.
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