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Transcriptome analysis of two inflorescence branching mutants reveals cytokinin is an important regulator in controlling inflorescence architecture in the woody plant Jatropha curcas
Chen, Mao-Sheng; Zhao, Mei-Li; Wang, Gui-Juan; He, Hui-Ying; Bai, Xue12; Pan, Bang-Zhen; Fu, Qian-Tang; Tao, Yan-Bin; Tang, Ming-Yong; Martinez-Herrera, Jorge; Xu, Zeng-Fu
2019
Source PublicationBMC PLANT BIOLOGY
ISSN1471-2229
Volume19Issue:1Pages:x
Abstract

Background In higher plants, inflorescence architecture is an important agronomic trait directly determining seed yield. However, little information is available on the regulatory mechanism of inflorescence development in perennial woody plants. Based on two inflorescence branching mutants, we investigated the transcriptome differences in inflorescence buds between two mutants and wild-type (WT) plants by RNA-Seq to identify the genes and regulatory networks controlling inflorescence architecture in Jatropha curcas L., a perennial woody plant belonging to Euphorbiaceae. Results Two inflorescence branching mutants were identified in germplasm collection of Jatropha. The duo xiao hua (dxh) mutant has a seven-order branch inflorescence, and the gynoecy (g) mutant has a three-order branch inflorescence, while WT Jatropha has predominantly four-order branch inflorescence, occasionally the three- or five-order branch inflorescences in fields. Using weighted gene correlation network analysis (WGCNA), we identified several hub genes involved in the cytokinin metabolic pathway from modules highly associated with inflorescence phenotypes. Among them, Jatropha ADENOSINE KINASE 2 (JcADK2), ADENINE PHOSPHORIBOSYL TRANSFERASE 1 (JcAPT1), CYTOKININ OXIDASE 3 (JcCKX3), ISOPENTENYLTRANSFERASE 5 (JcIPT5), LONELY GUY 3 (JcLOG3) and JcLOG5 may participate in cytokinin metabolic pathway in Jatropha. Consistently, exogenous application of cytokinin (6-benzyladenine, 6-BA) on inflorescence buds induced high-branch inflorescence phenotype in both low-branch inflorescence mutant (g) and WT plants. These results suggested that cytokinin is an important regulator in controlling inflorescence branching in Jatropha. In addition, comparative transcriptome analysis showed that Arabidopsis homologous genes Jatropha AGAMOUS-LIKE 6 (JcAGL6), JcAGL24, FRUITFUL (JcFUL), LEAFY (JcLFY), SEPALLATAs (JcSEPs), TERMINAL FLOWER 1 (JcTFL1), and WUSCHEL-RELATED HOMEOBOX 3 (JcWOX3), were differentially expressed in inflorescence buds between dxh and g mutants and WT plants, indicating that they may participate in inflorescence development in Jatropha. The expression of JcTFL1 was downregulated, while the expression of JcLFY and JcAP1 were upregulated in inflorescences in low-branch g mutant. Conclusions Cytokinin is an important regulator in controlling inflorescence branching in Jatropha. The regulation of inflorescence architecture by the genes involved in floral development, including TFL1, LFY and AP1, may be conservative in Jatropha and Arabidopsis. Our results provide helpful information for elucidating the regulatory mechanism of inflorescence architecture in Jatropha.

KeywordBranching Cytokinin Inflorescence Physic nut WGCNA analysis
Subject AreaPlant Sciences
DOI10.1186/s12870-019-2069-3
Indexed BySCI
Language英语
WOS IDWOS:000494814000005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11549
Collection能源植物分子育种研究组
Affiliation1.[Chen, Mao-Sheng
2.Zhao, Mei-Li
3.Wang, Gui-Juan
4.He, Hui-Ying
5.Bai, Xue
6.Pan, Bang-Zhen
7.Fu, Qian-Tang
8.Tao, Yan-Bin
9.Tang, Ming-Yong
10.Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China
11.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Mengla 666303, Yunnan, Peoples R China
12.[Zhao, Mei-Li
13.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
14.Campo Expt Huimanguillo, Inst Nacl Invest Forestales Agr & Pecuarias, Huimanguillo, Tabasco, Mexico
Recommended Citation
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Chen, Mao-Sheng,Zhao, Mei-Li,Wang, Gui-Juan,et al. Transcriptome analysis of two inflorescence branching mutants reveals cytokinin is an important regulator in controlling inflorescence architecture in the woody plant Jatropha curcas[J]. BMC PLANT BIOLOGY,2019,19(1):x.
APA Chen, Mao-Sheng.,Zhao, Mei-Li.,Wang, Gui-Juan.,He, Hui-Ying.,Bai, Xue.,...&Xu, Zeng-Fu.(2019).Transcriptome analysis of two inflorescence branching mutants reveals cytokinin is an important regulator in controlling inflorescence architecture in the woody plant Jatropha curcas.BMC PLANT BIOLOGY,19(1),x.
MLA Chen, Mao-Sheng,et al."Transcriptome analysis of two inflorescence branching mutants reveals cytokinin is an important regulator in controlling inflorescence architecture in the woody plant Jatropha curcas".BMC PLANT BIOLOGY 19.1(2019):x.
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