XTBG OpenIR  > 植物生理生态研究组
Processes controlling programmed cell death of root velamen radicum in an epiphytic orchid
Li, Jia-Wei; Zhang, Shi-Bao; Xi, Hui-Peng; Bradshaw, Corey J. A.; Zhang, Jiao-Lin
2020
Source PublicationANNALS OF BOTANY
ISSN0305-7364
Volume126Issue:2Pages:261-275
Abstract

Background and Aims Development of the velamen radicum on the outer surface of the root epidermis is an important characteristic for water uptake and retention in some plant families, particularly epiphytic orchids, for survival under water-limited environments. Velamen radicum cells derive from the primary root meristem; however, following this development, velamen radicum cells die by incompletely understood processes of programmed cell death (PCD). Methods We combined the use of transmission electron microscopy, X-ray micro-tomography and transcriptome methods to characterize the major anatomical and molecular changes that occur during the development and death of velamen radicum cells of Cymbidium tract' mum, a typical epiphytic orchid, to determine how PCD occurs. Key Results Typical changes of PCD in anatomy and gene expression were observed in the development of velamen radicum cells. During the initiation of PCD, we found that both cell and vacuole size increased, and several genes involved in brassinosteroid and ethylene pathways were upregulated. In the stage of secondary cell wall formation, significant anatomical changes included DNA degradation, cytoplasm thinning, organelle decrease, vacuole rupture and cell wall thickening. Changes were found in the expression of genes related to the biosynthesis of cellulose and lignin. which are instrumental in the formation of secondary cell walls, and are regulated by cytoskeleton-related factors and phenylalanine ammonia-lyase. In the final stage of PCI), cell autolysis was terminated from the outside to the inside of the velamen radicum. The regulation of genes related to autophagy, vacuolar processing enzyme. cysteine proteases and metacaspase was involved in the final execution of cell death and autolysis. Conclusions Our results showed that the development of the root velamen radicum in an epiphytic orchid was controlled by the process of PCD, which included initiation of PCD, followed by formation of the secondary cell wall, and execution of autolysis following cell death.

KeywordEpiphytic orchids programmed cell death (PCD) root tip velamen radicum X-ray computerized tomography transmission electron microscopy
Subject AreaPlant Sciences
DOI10.1093/aob/mcaa077
Indexed BySCI
Language英语
WOS IDWOS:000561731700006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11786
Collection植物生理生态研究组
Affiliation1.[Li, Jia-Wei
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Forest Ecol, Mengla 666303, Yunnan, Peoples R China
3.Chinese Acad Sci, Ctr Plant Ecol, Core Bot Gardens, Mengla 666303, Yunnan, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China
5.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Hort Dept, Mengla 666303, Yunnan, Peoples R China
6.[Bradshaw, Corey J. A.] Flinders Univ S Australia, Coll Sci & Engn, Global Ecol, GPO Box 2100, Adelaide, SA 5001, Australia
Recommended Citation
GB/T 7714
Li, Jia-Wei,Zhang, Shi-Bao,Xi, Hui-Peng,et al. Processes controlling programmed cell death of root velamen radicum in an epiphytic orchid[J]. ANNALS OF BOTANY,2020,126(2):261-275.
APA Li, Jia-Wei,Zhang, Shi-Bao,Xi, Hui-Peng,Bradshaw, Corey J. A.,&Zhang, Jiao-Lin.(2020).Processes controlling programmed cell death of root velamen radicum in an epiphytic orchid.ANNALS OF BOTANY,126(2),261-275.
MLA Li, Jia-Wei,et al."Processes controlling programmed cell death of root velamen radicum in an epiphytic orchid".ANNALS OF BOTANY 126.2(2020):261-275.
Files in This Item: Download All
File Name/Size DocType Version Access License
Processes controllin(1648KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Jia-Wei]'s Articles
[Zhang, Shi-Bao]'s Articles
[Xi, Hui-Peng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Jia-Wei]'s Articles
[Zhang, Shi-Bao]'s Articles
[Xi, Hui-Peng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Jia-Wei]'s Articles
[Zhang, Shi-Bao]'s Articles
[Xi, Hui-Peng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Processes controlling programmed cell death of root velamen radicum in an epiphytic orchid.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.