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Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula
Guo, Shiqi1; Zhang, Xiaojia1; Bai, Quanzi1; Zhao, Weiyue1; Fang, Yuegenwang1; Zhou, Shaoli1; Zhao, Baolin; He, Liangliang1; Chen, Jianghua
2020
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume21Issue:14Pages:-
AbstractPlant height is a vital agronomic trait that greatly determines crop yields because of the close relationship between plant height and lodging resistance. Legumes play a unique role in the worldwide agriculture; however, little attention has been given to the molecular basis of their height. Here, we characterized the first dwarf mutantmini plant 1(mnp1) of the model legume plantMedicago truncatula. Our study found that both cell length and the cell number of internodes were reduced in amnp1mutant. Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned theMNP1gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. MNP1 was highly homologous toPisum sativumLS. The subcellular localization showed that MNP1 was located in the chloroplast. Further analysis indicated that GA(3)could significantly restore the plant height ofmnp1-1, and expression ofMNP1in acps1mutant ofArabidopsispartially rescued its mini-plant phenotype, indicating the conservation function of MNP1 in GA biosynthesis. Our results provide valuable information for understanding the genetic regulation of plant height inM. truncatula.
Keyworddwarfism gene cloning MNP1 CPS Medicago truncatula
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.3390/ijms21144968
Indexed BySCI
Language英语
WOS IDWOS:000554084400001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/11793
Collection2012年后新成立研究组
Affiliation1.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, 88 Xuefu Rd, Kunming 650223, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo, Shiqi,Zhang, Xiaojia,Bai, Quanzi,et al. Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(14):-.
APA Guo, Shiqi.,Zhang, Xiaojia.,Bai, Quanzi.,Zhao, Weiyue.,Fang, Yuegenwang.,...&Chen, Jianghua.(2020).Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(14),-.
MLA Guo, Shiqi,et al."Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.14(2020):-.
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