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Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (JcDGAT1 and JcDGAT2) Enhances Oil Production in the Woody Perennial Biofuel Plant Jatropha curcas
Zhang, Tian-Tian; He, Huiying; Xu, Chuan-Jia; Fu, Qiantang; Tao, Yan-Bin; Xu, Ronghua3; Xu, Zeng-Fu
2021
Source PublicationPLANTS-BASEL
ISSN2223-7747
Volume10Issue:4Pages:-
Abstract

Diacylglycerol acyltransferase (DGAT) is the only enzyme that catalyzes the acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol (DAG) to form triacylglycerol (TAG). The two main types of DGAT enzymes in the woody perennial biofuel plant Jatropha curcas, JcDGAT1 and JcDGAT2, were previously characterized only in heterologous systems. In this study, we investigated the functions of JcDGAT1 and JcDGAT2 in J. curcas. JcDGAT1 and JcDGAT2 were found to be predominantly expressed during the late stages of J. curcas seed development, in which large amounts of oil accumulated. As expected, overexpression of JcDGAT1 or JcDGAT2 under the control of the CaMV35S promoter gave rise to an increase in seed kernel oil production, reaching a content of 53.7% and 55.7% of the seed kernel dry weight, respectively, which were respectively 25% and 29.6% higher than that of control plants. The increase in seed oil content was accompanied by decreases in the contents of protein and soluble sugars in the seeds. Simultaneously, there was a two- to four-fold higher leaf TAG content in transgenic plants than in control plants. Moreover, by analysis of the fatty acid (FA) profiles, we found that JcDGAT1 and JcDGAT2 had the same substrate specificity with preferences for C18:2 in seed TAGs, and C16:0, C18:0, and C18:1 in leaf TAGs. Therefore, our study confirms the important role of JcDGAT1 and JcDGAT2 in regulating oil production in J. curcas.

KeywordCanopy openness Diversity indices Microenvironment Rarity Soil nutrients Soil pH Subtropical forest Similarity Topography
Subject AreaBiodiversity & Conservation ; Environmental Sciences & Ecology
DOI10.1016/j.gecco.2020.e01443
Indexed BySCI
Language英语
WOS IDWOS:000641412100012
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/12261
Collection能源植物分子育种研究组
Affiliation1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Plant Resources & Sustainable Us, Menglun 666303, Mengla, Peoples R China
3.Chinese Acad Sci, Core Bot Gardens, Ctr Econ Bot, Menglun 666303, Mengla, Peoples R China
4.Anhui Sci & Technol Univ, Coll Life & Hlth Sci, Fengyang 233100, Peoples R China
5.Guangxi Univ, Coll Forestry, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Tian-Tian,He, Huiying,Xu, Chuan-Jia,et al. Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (JcDGAT1 and JcDGAT2) Enhances Oil Production in the Woody Perennial Biofuel Plant Jatropha curcas[J]. PLANTS-BASEL,2021,10(4):-.
APA Zhang, Tian-Tian.,He, Huiying.,Xu, Chuan-Jia.,Fu, Qiantang.,Tao, Yan-Bin.,...&Xu, Zeng-Fu.(2021).Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (JcDGAT1 and JcDGAT2) Enhances Oil Production in the Woody Perennial Biofuel Plant Jatropha curcas.PLANTS-BASEL,10(4),-.
MLA Zhang, Tian-Tian,et al."Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (JcDGAT1 and JcDGAT2) Enhances Oil Production in the Woody Perennial Biofuel Plant Jatropha curcas".PLANTS-BASEL 10.4(2021):-.
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