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Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts
Frangedakis, Eftychios; Guzman-Chavez, Fernando; Rebmann, Marius; Markel, Kasey1; Yu, Ying2,3; Perraki, Artemis4; Tse, Sze Wai; Liu, Yang5,6; Rever, Jenna; Sauret-Gueto, Susanna; Goffinet, Bernard8; Schneider, Harald9; Haseloff, Jim
AbstractY Chloroplasts are attractive platforms for synthetic biology applications since they are capable of driving very high levels of transgene expression, if mRNA production and stability are properly regulated. However, plastid transformation is a slow process and currently limited to a few plant species. The liverwort Marchantia polymorpha is a simple model plant that allows rapid transformation studies; however, its potential for protein hyper-expression has not been fully exploited. This is partially due to the fact that chloroplast post-transcriptional regulation is poorly characterized in this plant. We have mapped patterns of transcription in Marchantia chloroplasts. Furthermore, we have obtained and compared sequences from 51 bryophyte species and identified putative sites for pentatricopeptide repeat protein binding that are thought to play important roles in mRNA stabilization. Candidate binding sites were tested for their ability to confer high levels of reporter gene expression in Marchantia chloroplasts, and levels of protein production and effects on growth were measured in homoplastic transformed plants. We have produced novel DNA tools for protein hyperexpression in this facile plant system that is a test-bed for chloroplast engineering.
KeywordMarchantia plant chloroplast plastome transcriptome gene assembly
Subject AreaBiochemistry & Molecular Biology
Indexed BySCI
WOS IDWOS:000674933400009
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Document Type期刊论文
Affiliation1.Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England
2.Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA
3.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 311121, Peoples R China
4.Huangshan Univ, Coll Life & Environm Sci, Huangshan 245041, Peoples R China
5.Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion 70013, Crete, Greece
6.Fairy Lake Bot Garden, Shenzhen 518004, Guangdong, Peoples R China
7.Chinese Acad Sci, Shenzhen 518004, Guangdong, Peoples R China
8.Univ Cambridge, Crop Sci Ctr, 93 Lawrence Weaver Rd, Cambridge CB3 0LE, England
9.Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA
10.Chinese Acad Sci, Ctr Integrat Conservat, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Frangedakis, Eftychios,Guzman-Chavez, Fernando,Rebmann, Marius,et al. Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts[J]. ACS SYNTHETIC BIOLOGY,2021,10(7):1651-1666.
APA Frangedakis, Eftychios.,Guzman-Chavez, Fernando.,Rebmann, Marius.,Markel, Kasey.,Yu, Ying.,...&Haseloff, Jim.(2021).Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts.ACS SYNTHETIC BIOLOGY,10(7),1651-1666.
MLA Frangedakis, Eftychios,et al."Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts".ACS SYNTHETIC BIOLOGY 10.7(2021):1651-1666.
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