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A single-step isolation of squalene from olive oil deodorizer distillates by using centrifugal partition chromatography
Xynos, Nikos; Zervos, Michail; Angelis, Apostolis; Aligiannis, Nektarios; Skaltsounis, Alexios-Leandros
2016
Source PublicationSEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
Volume51Issue:5Pages:830-835
AbstractHigh added-value squalene (SQ) was purified in one step from olive oil deodorizer distillates (OODD) using preparative centrifugal partition chromatography (CPC) method, operating in the dual mode. The fractionation was performed using a non-aqueous biphasic solvent system consisting of heptane-acetonitrile-butanol (1.8:1.4:0.7, v/v/v), leading to the isolation of the target compound in 4 hours, using a preparative 1L column. Furthermore, a fast UHPLC-DAD method was developed and applied for the identification and quantification of SQ in both OODD and purified form. The content of SQ in the initial material was 23.4%, while the purity of the isolated SQ was 95.5%. The recovery of SQ was calculated at 76.3%. The productivity of the process was calculated at 234 mg/h/L.
KeywordCounter-current Chromatography Extraction Components Separation Recovery Carbon Sebum
Subject AreaChemistry ; Engineering
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9741
Collection文献共享
Recommended Citation
GB/T 7714
Xynos, Nikos,Zervos, Michail,Angelis, Apostolis,et al. A single-step isolation of squalene from olive oil deodorizer distillates by using centrifugal partition chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2016,51(5):830-835.
APA Xynos, Nikos,Zervos, Michail,Angelis, Apostolis,Aligiannis, Nektarios,&Skaltsounis, Alexios-Leandros.(2016).A single-step isolation of squalene from olive oil deodorizer distillates by using centrifugal partition chromatography.SEPARATION SCIENCE AND TECHNOLOGY,51(5),830-835.
MLA Xynos, Nikos,et al."A single-step isolation of squalene from olive oil deodorizer distillates by using centrifugal partition chromatography".SEPARATION SCIENCE AND TECHNOLOGY 51.5(2016):830-835.
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