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Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution
Xu, Mingfeng; Zhu, Qin; Wang, Huizhong; Du, Qizhen; Xu, Maojun
2016
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume39Issue:3Pages:139-144
AbstractHigh-speed counter-current chromatography (HSCCC) was successfully used for the separation of abietane-type diterpenoids from the medicinal plant C. kaichianum, which were not separated in our previous study using preparative HPLC. The HSCCC separation employed the lower phases of n-hexane-ethyl acetate-methanol-water (HEMW) 4:5:4:5 and HEMW 4:5:5:4 as the mobile phase for stepwise elution while the upper phase of HEMW 4:5:4:5 was used as the stationary phase. HSCCC separation yielded 90.5mg of compound 1(kaichianone A), 137.7mg of compound 2 (kaichianone B), 125.0mg of compound 3 (teuvincenone E), and 227.6mg of compound 4 (taxusabietane A) with purities of 95.3%, 97.2%, 97.8%, and 98.6%, respectively, as determined by HPLC. Compounds 1-2 are two new abietane-type diterpenoids while Compounds 3-4 are known abietane-type diterpenoids, analyzed by ESIMS and NMR data. The results demonstrated that HSCCC can be an excellent alternative for other separation methods. The two new compounds showed significant cytotoxicity against ileocecal carcinoma HCT-8 and breast adenocarcinoma MCF-7 cells.
KeywordPetas.tes.s. Moore Purification Root Mandarinorum Delivery Growth Bungei Assay
Subject AreaBiochemistry & Molecular Biology ; Chemistry
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9743
Collection文献共享
Recommended Citation
GB/T 7714
Xu, Mingfeng,Zhu, Qin,Wang, Huizhong,et al. Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2016,39(3):139-144.
APA Xu, Mingfeng,Zhu, Qin,Wang, Huizhong,Du, Qizhen,&Xu, Maojun.(2016).Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,39(3),139-144.
MLA Xu, Mingfeng,et al."Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 39.3(2016):139-144.
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