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Harnessing the power of RADseq for ecological and evolutionary genomics
Andrews, Kimberly R.; Good, Jeffrey M.; Miller, Michael R.; Luikart, Gordon; Hohenlohe, Paul A.
2016
Source PublicationNATURE REVIEWS GENETICS
ISSN1471-0056
Volume17Issue:2Pages:81-92
AbstractHigh-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are enabling the low-cost discovery and genotyping of thousands of genetic markers for any species, including non-model organisms, which is revolutionizing ecological, evolutionary and conservation genetics. Technical differences among these methods lead to important considerations for all steps of genomics studies, from the specific scientific questions that can be addressed, and the costs of library preparation and sequencing, to the types of bias and error inherent in the resulting data. In this Review, we provide a comprehensive discussion of RADseq methods to aid researchers in choosing among the many different approaches and avoiding erroneous scientific conclusions from RADseq data, a problem that has plagued other genetic marker types in the past.
KeywordGeneration Sequencing Data Next-generation Population Genomics Reduced-representation Snp Discovery Rna-seq Ultraconserved Elements Westslope Cutthroat Adaptive Radiation Natural-selection
Subject AreaGenetics & Heredity
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9747
Collection文献共享
Recommended Citation
GB/T 7714
Andrews, Kimberly R.,Good, Jeffrey M.,Miller, Michael R.,et al. Harnessing the power of RADseq for ecological and evolutionary genomics[J]. NATURE REVIEWS GENETICS,2016,17(2):81-92.
APA Andrews, Kimberly R.,Good, Jeffrey M.,Miller, Michael R.,Luikart, Gordon,&Hohenlohe, Paul A..(2016).Harnessing the power of RADseq for ecological and evolutionary genomics.NATURE REVIEWS GENETICS,17(2),81-92.
MLA Andrews, Kimberly R.,et al."Harnessing the power of RADseq for ecological and evolutionary genomics".NATURE REVIEWS GENETICS 17.2(2016):81-92.
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