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Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe
Ji, Wei; Du, Lingyun; Zhang, Yuanfu; Liu, Guofu; Wang, Shuhao
2015
Source PublicationFOOD ANALYTICAL METHODS
ISSN1936-9751
Volume8Issue:10Pages:2596-2604
AbstractAn ultrasensitive fluorescence immunoassay was developed for detection of bisphenol A (BPA). Herein, thiol-modified single-strand DNA (SH-ssDNAs) and anti-BPA antibodies were simultaneously conjugated with gold nanoparticles (AuNPs), and the fluorescein isothiocyanate-labeled single-strand DNA was hybridized with SH-ssDNAs to form a dual-codified probe. In the presence of BPA, a competitive immunoreaction was conducted between BPA and BPA coating antigens immobilized on microplate for the anti-BPA antibodies of dual-codified probe. Then, in order to avoid the fluorescent signals quenched by AuNPs, restriction enzyme BamH D dagger was employed to cut the fluorescent labels from immunocomplex, resulting in an increase of fluorescence emission. Under optimized conditions, the linear range for detection of BPA is from 1.0 x 10(-2) to 1.0 x 10(3) ng/L with a low limit of detection of 3.4 x 10(-3) ng/L. This method is of good sensibility, stability, and specificity, and it can be used to detect BPA in actual milk products and its migration from packaging materials into milk.
KeywordLinked-immunosorbent-assay Tandem Mass-spectrometry Human Exposure Migration Food Bpa Samples Dna Temperature Biosensor
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9803
Collection文献共享
Recommended Citation
GB/T 7714
Ji, Wei,Du, Lingyun,Zhang, Yuanfu,et al. Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe[J]. FOOD ANALYTICAL METHODS,2015,8(10):2596-2604.
APA Ji, Wei,Du, Lingyun,Zhang, Yuanfu,Liu, Guofu,&Wang, Shuhao.(2015).Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe.FOOD ANALYTICAL METHODS,8(10),2596-2604.
MLA Ji, Wei,et al."Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe".FOOD ANALYTICAL METHODS 8.10(2015):2596-2604.
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