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title: Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe
author: Ji, Wei;  Du, Lingyun;  Zhang, Yuanfu;  Liu, Guofu;  Wang, Shuhao
Issued Date: 2015
Abstract: An ultrasensitive fluorescence immunoassay was developed for detection of bisphenol A (BPA). Herein, thiol-modified single-strand DNA (SH-ssDNAs) and anti-BPA antibodies were simultaneously conjugated with gold nanoparticles (AuNPs), and the fluorescein isothiocyanate-labeled single-strand DNA was hybridized with SH-ssDNAs to form a dual-codified probe. In the presence of BPA, a competitive immunoreaction was conducted between BPA and BPA coating antigens immobilized on microplate for the anti-BPA antibodies of dual-codified probe. Then, in order to avoid the fluorescent signals quenched by AuNPs, restriction enzyme BamH D dagger was employed to cut the fluorescent labels from immunocomplex, resulting in an increase of fluorescence emission. Under optimized conditions, the linear range for detection of BPA is from 1.0 x 10(-2) to 1.0 x 10(3) ng/L with a low limit of detection of 3.4 x 10(-3) ng/L. This method is of good sensibility, stability, and specificity, and it can be used to detect BPA in actual milk products and its migration from packaging materials into milk.
Source: FOOD ANALYTICAL METHODS
Related URLs: 10.1007/s12161-015-0162-2
Appears in Collections:文献共享_期刊论文

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Recommended Citation:
Ji, Wei,Du, Lingyun,Zhang, Yuanfu,et al. Ultrasensitive Fluorescence Immunoassay For Detection Of Bisphenol A In Milk Products Using Functionalized Gold Nanoparticles As Probe[J]. Food Analytical Methods,2015,8(10):2596-2604.

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