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title: Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe
author: Ji, Wei;  Du, Lingyun;  Zhang, Yuanfu;  Liu, Guofu;  Wang, Shuhao
Issued Date: 2015
Abstract: An ultrasensitive fluorescence immunoassay was developed for detection of bisphenol A (BPA). Herein, thiol-modified single-strand DNA (SH-ssDNAs) and anti-BPA antibodies were simultaneously conjugated with gold nanoparticles (AuNPs), and the fluorescein isothiocyanate-labeled single-strand DNA was hybridized with SH-ssDNAs to form a dual-codified probe. In the presence of BPA, a competitive immunoreaction was conducted between BPA and BPA coating antigens immobilized on microplate for the anti-BPA antibodies of dual-codified probe. Then, in order to avoid the fluorescent signals quenched by AuNPs, restriction enzyme BamH D dagger was employed to cut the fluorescent labels from immunocomplex, resulting in an increase of fluorescence emission. Under optimized conditions, the linear range for detection of BPA is from 1.0 x 10(-2) to 1.0 x 10(3) ng/L with a low limit of detection of 3.4 x 10(-3) ng/L. This method is of good sensibility, stability, and specificity, and it can be used to detect BPA in actual milk products and its migration from packaging materials into milk.
Related URLs: 10.1007/s12161-015-0162-2
Appears in Collections:文献共享_期刊论文

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Recommended Citation:
Ji, Wei,Du, Lingyun,Zhang, Yuanfu,et al. Ultrasensitive Fluorescence Immunoassay For Detection Of Bisphenol A In Milk Products Using Functionalized Gold Nanoparticles As Probe[J]. Food Analytical Methods,2015,8(10):2596-2604.

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