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A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances
Hein, Marco Y.; Hubner, Nina C.; Poser, Ina; Cox, Juergen; Nagaraj, Nagarjuna; Toyoda, Yusuke; Gak, Igor A.; Weisswange, Ina; Mansfeld, Joerg; Buchholz, Frank; Hyman, Anthony A.; Mann, Matthias
2015
Source PublicationCELL
ISSN0092-8674
Volume163Issue:3Pages:712-723
AbstractThe organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins under near-endogenous control, which we used as input for a next-generation interaction survey. Using quantitative proteomics, we detect specific interactions, estimate interaction stoichiometries, and measure cellular abundances of interacting proteins. These three quantitative dimensions reveal that the protein network is dominated by weak, substoichiometric interactions that play a pivotal role in defining network topology. The minority of stable complexes can be identified by their unique stoichiometry signature. This study provides a rich interaction dataset connecting thousands of proteins and introduces a framework for quantitative network analysis.
KeywordProtein Interaction Network Mass-spectrometry Saccharomyces-cerevisiae Bac Transgeneomics Copy-number Scale Map Complexes Reveals Yeast Accurate
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9804
Collection文献共享
Recommended Citation
GB/T 7714
Hein, Marco Y.,Hubner, Nina C.,Poser, Ina,et al. A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances[J]. CELL,2015,163(3):712-723.
APA Hein, Marco Y..,Hubner, Nina C..,Poser, Ina.,Cox, Juergen.,Nagaraj, Nagarjuna.,...&Mann, Matthias.(2015).A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.CELL,163(3),712-723.
MLA Hein, Marco Y.,et al."A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances".CELL 163.3(2015):712-723.
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