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Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer
Du, Lingyun; Zhang, Chunyan; Wang, Longjun; Liu, Guofu; Zhang, Yuanfu; Wang, Shuhao
2015
Source PublicationMICROCHIMICA ACTA
ISSN0026-3672
Volume182Issue:42433Pages:539-545
AbstractWe demonstrate an ultrasensitive time-resolved fluorescence immunoassay for bisphenol A (BPA) where gold nanoparticles (Au-NPs) were modified with anti-BPA antibody and thiolated dsDNA-biotin acting as a signal amplifier. In a competitive reaction, the analyte (BPA) competes with immobilized BPA-ovalbumin conjugate on the surface of microtiter plates to bind to the anti-BPA antibodies on the surface of the Au-NPs. In the next step, a Eu(III)-labeled streptavidin is added to link to the SH-dsDNA-biotin as a tracer. Fluorescence is amplified via both the Au-NPs and the biotin-streptavidin systems, and its intensity is measured in a time-resolved fluorescence immunoassay. The linear range is from 1.0 fga (TM) mL(-1) to 1.0 nga (TM) mL(-1), and the detection limit is 0.3 fg mL(-1), respectively. The method was applied to the determination of BPA in spiked water sample, with recoveries ranging from 90.2 to 106.4 %.
KeywordTandem Mass-spectrometry Liquid-liquid Microextraction Exposure Risk Extraction Expression Proteins Quechers Cancer Signal
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9812
Collection文献共享
Recommended Citation
GB/T 7714
Du, Lingyun,Zhang, Chunyan,Wang, Longjun,et al. Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer[J]. MICROCHIMICA ACTA,2015,182(42433):539-545.
APA Du, Lingyun,Zhang, Chunyan,Wang, Longjun,Liu, Guofu,Zhang, Yuanfu,&Wang, Shuhao.(2015).Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer.MICROCHIMICA ACTA,182(42433),539-545.
MLA Du, Lingyun,et al."Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer".MICROCHIMICA ACTA 182.42433(2015):539-545.
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