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title: Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer
author: Du, Lingyun;  Zhang, Chunyan;  Wang, Longjun;  Liu, Guofu;  Zhang, Yuanfu;  Wang, Shuhao
Issued Date: 2015
Abstract: We demonstrate an ultrasensitive time-resolved fluorescence immunoassay for bisphenol A (BPA) where gold nanoparticles (Au-NPs) were modified with anti-BPA antibody and thiolated dsDNA-biotin acting as a signal amplifier. In a competitive reaction, the analyte (BPA) competes with immobilized BPA-ovalbumin conjugate on the surface of microtiter plates to bind to the anti-BPA antibodies on the surface of the Au-NPs. In the next step, a Eu(III)-labeled streptavidin is added to link to the SH-dsDNA-biotin as a tracer. Fluorescence is amplified via both the Au-NPs and the biotin-streptavidin systems, and its intensity is measured in a time-resolved fluorescence immunoassay. The linear range is from 1.0 fga (TM) mL(-1) to 1.0 nga (TM) mL(-1), and the detection limit is 0.3 fg mL(-1), respectively. The method was applied to the determination of BPA in spiked water sample, with recoveries ranging from 90.2 to 106.4 %.
Related URLs: 10.1007/s00604-014-1356-9
Appears in Collections:文献共享_期刊论文

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Recommended Citation:
Du, Lingyun,Zhang, Chunyan,Wang, Longjun,et al. Ultrasensitive Time-resolved Microplate Fluorescence Immunoassay For Bisphenol A Using A System Composed On Gold Nanoparticles And A Europium(iii)-labeled Streptavidin Tracer[J]. Microchimica Acta,2015,182(42433):539-545.

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