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The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Beng, Kingsly Chuo; Tomlinson, Kyle W.; Shen, Xian Hui
2016
Source PublicationSCIENTIFIC REPORTS
Volume6Issue:xPages:24965
AbstractMetabarcoding potentially offers a rapid and cheap method of monitoring biodiversity, but real-world applications are few. We investigated its utility in studying patterns of litter arthropod diversity and composition in the tropics. We collected litter arthropods from 35 matched forest-plantation sites across Xishuangbanna, southwestern China. A new primer combination and the MiSeq platform were used to amplify and sequence a wide variety of litter arthropods using simulated and real-world communities. Quality filtered reads were clustered into 3,624 MOTUs at >= 97% similarity and the taxonomy of each MOTU was predicted. We compared diversity and compositional differences between forests and plantations (rubber and tea) for all MOTUs and for eight arthropod groups. We obtained similar to 100% detection rate after in silico sequencing six mock communities with known arthropod composition. Ordination showed that rubber, tea and forest communities formed distinct clusters. a-diversity declined significantly between forests and adjacent plantations for more arthropod groups in rubber than tea, and diversity of order Orthoptera increased significantly in tea. Turnover was higher in forests than plantations, but patterns differed among groups. Metabarcoding is useful for quantifying diversity patterns of arthropods under different land-uses and the MiSeq platform is effective for arthropod metabarcoding in the tropics.
KeywordBeta-diversity Forest Vegetation Leaf-litter Sw China Soil Dna Biodiversity Xishuangbanna Conservation Communities Indicator
Document Type期刊论文
Identifierhttp://ir.xtbg.org.cn/handle/353005/9879
Collection2012年后新成立研究组
Recommended Citation
GB/T 7714
Beng, Kingsly Chuo,Tomlinson, Kyle W.,Shen, Xian Hui. The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics[J]. SCIENTIFIC REPORTS,2016,6(x):24965.
APA Beng, Kingsly Chuo,Tomlinson, Kyle W.,&Shen, Xian Hui.(2016).The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics.SCIENTIFIC REPORTS,6(x),24965.
MLA Beng, Kingsly Chuo,et al."The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics".SCIENTIFIC REPORTS 6.x(2016):24965.
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